Effects of Chenopodiummurale Linn. Leaf Extract on Blood Glucose, Lipid Profile and Liver Enzymes in Streptozotocin-induced Diabetic Mice

Title: Effects of Chenopodiummurale Linn. Leaf Extract on Blood Glucose, Lipid Profile and Liver Enzymes in Streptozotocin-induced Diabetic Mice Miss.Nuzhat Tasnim1, SyedaFaizaebnat Maisha1, NazmunNahar Tamanna1,A. K. M. Shafiur Rahman1,Monalisa Monwar1, Tarannum Naz1,Mir Imam Ibne Wahed2 1Department of Pharmacy, Faculty of Science, Varendra University, Rajshahi-6204, Bangladesh. 2Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi -6205, Bangladesh. Correspondence Address: Dr. Mir Imam IbneWahed Professor,Department of Pharmacy, Faculty of Science, University of Rajshahi Rajshahi 6205, Bangladesh. Cell:+8801718133894, Email: drmirimam@gmail.com Background: Diabetes mellitus is a major endocrine disorder characterized byhyperglycemia, abnormal lipid and protein metabolism and associated with long-term microvascular and macrovascular complications.The plant Chenopodiummurale Linn.hasdemonstrated anti-inflammatory, antioxidant and hepatoprotective properties.The current study was conducted to evaluate the effect ofC. muraleleaf(CML) extract in diabetic mice. Methods: Normal mice were subjected to acute toxicity test and OGTT. Diabetes was induced by intraperitinealinjection of streptozotocin(45mg/kg) in mice. Diabetic mice were treated with CML extract (200 and 400mg/kg),metformin (150mg/kg) and/or vehicle (0.5% MC)for the period of 15 days and healthy mice was used as nomal control.Time course of changes in blood glucose level and body weight of mice were measured. Serum was taken to estimate the levels of cholesterol (TC), triglycerides (TG), low-density-lipoprotein (LDL), high-density-lipoprotein (HDL), alanine transaminase (ALT) and aspartate transaminase (AST). Results:The CML extract was found to be safe at1000mg/kg and improved glucose tolerance in normal mice. The survival rate was significantly higher and body weight was restored at high dose when compared with diabetic mice.The CML extracts(200 and 400 mg/kg)exhibitedsignificant attenuation of glucose levels and the effect was dose-dependent. In diabetic mice elevated TC,TGand LDL were decreased and low level of HDL was increased by the treatment. Moreover, CML extractreduced ALT and AST level dose-dependently. Conclusion:We concluded that methanolic extract of C. murale leaf hasglucose and lipid lowering efficacy and protected liver function in diabetic mice. Key Words:Chenopodiummurale,blood glucose, lipid profile, liver enzymes, diabetic mice